Sentence examples for blots were stained with from inspiring English sources

For detection of PHLDA1 expression, blots were stained with 1 1000 dilution of anti-PHLDA1 antibody (EPR6674, abcam®).

For detection of PDK4 expression, blots were stained with 2 µg/mL dilution of anti-PDK4 (Novus07049, Novus Biologicals).

Blots were stained with antibodies against EGFR (D38B1), HER2/ERRB2 (D8F12), HER3/ERRB3(D22C5), and HER4/ERBB4 (111B2),and GAPDH (D16H11) purchased from Cell Signaling Technology and used a dilution of 1 1000 in 5% dry milk in TBST for 1 h at 22 °C.

For visualization and spot isolation of plasminogen-binding proteins, blots were stained with India Ink.

The blots were stained with 0.2% Ponceau S red to ensure equal protein loading.

The blots were stained with antibodies directed against different epitopes of WASP.

To facilitate the comparative analysis of the different sets of 2D-blots probed with individual sera from BCP and healthy volunteers, the 2D-blots were stained with colloidal gold following the immunodetection step.

Following transfer, the blot was stained with Ponceau red to determine the efficiency of the transfer.

The blot was stained with Ponceau S to reveal bait input.

Samples were blotted in duplicate simultaneously; one blot was immediately incubated with monoclonal antibody to misfolded SOD1 (C4F6, MediMabs) diluted 1 250 in blocking buffer (TBS-T with 5% (w/v) nonfat dry milk); the duplicate blot was stained with Ponceau S in 5% acetic acid to visualize total protein loaded onto the membrane.

Blots were stained in parallel with Ponceau S (Sigma, St . Louis MO).