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Insert fragments to be used as probes for genomic DNA blots were separated from vector sequences on 0.7% agarose gels and extracted by electroelution [ 38].
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For Western blotting analysis and immunoprecipitation, cells were separated from seminal plasma by densitometry using a two-layer Percoll gradient (40 and 80%) as described previously [ 34] and were capacitated as described above.
In this case roots were washed for 10 min in ice-cold 5 mM CaCl2 solution to displace extracellular Cd [ 27], rinsed in distilled water and gently blotted with paper towels; shoots were separated from roots and the tissues were frozen in liquid N2 and stored at -80°C.
Equal amounts of proteins were separated from the different samples by SDS-PAGE, and blotted onto PVDF membranes.
The tumor cells were separated from Mo-MDSC after coculture, and RT-PCR and Western blotting were performed.
Eluted proteins were separated from beads by centrifugation, concentrated, and subjected to SDS-PAGE and Western blot.
Oxidized mRNAs (O) were separated from non-oxidized mRNAs (N) by IP with 15A3 antibodies and then analyzed by Southern blotting.
Proteins were separated from membrane lysates by SDS-PAGE, and GLR-1 GFP GLR-1 GFPere simultaneorsly detected by Western blotting using actinbination of anti-GFP antibodies (GeneTex Inc).
Many more were separated from loved ones.
For Western blot and RNA isolation, the cancerous tissue was separated from the surrounding normal thyroid fragments by expert evaluation of the pathologist.
With this technique, intact large extrachromosomal EBV plasmids can be separated from genomic DNA and detected by Southern blot hybridization.
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