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Blots were scanned using the LiCor Odyssey scanner and each channel simultaneously visualized and quantitated using the instrument software20.
Blots were scanned using the same scanner.
Blots were scanned using the program ImageQuant 5.2 (Molecular Dynamics).
Blots were scanned using the Typhoon scanner (GE Healthcare), and bands were analyzed using ImageQuant™ TL software.
For quantification, blots were scanned using the Typhoon 9400 (GE Healthcare Life Sciences) and analyzed using ImageQuantTL.
Blots were scanned using an Odyssey infrared imaging system (LI-COR), and proteins were quantitatively analyzed by the Odyssey software.
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Secondary antibody staining was done using Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLight 800 4X PEG and Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLight 680 at 1 10,000 for 2 h at RT. Blot was scanned using a LI-COR Odyssey scanner.
Each blot was scanned using a densitometer in order to obtain data for statistical analysis.
The resulting blot was scanned using the Odyssey infra-red scanner at 800 μm and results analyzed using the LI-COR Odyssey™ application software (version 3.0, LI-COR, Inc., Lincoln, NE, USA), quantifying the protein band intensities and normalizing with the respective β-tubulin intensities (as control).
Autoradiograms obtained from western blot analyses were scanned using Epson 1680 pro scanner and densitometric analysis was performed using Scion Image (Scion Corporation, MD).
All gels and western blot images were scanned using Epson Perfection V750 PRO and Adobe Photoshop software.
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