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Blots were reprobed using anti-alpha-tubulin (Sigma) antibodies.
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All blots were reprobed with anti- β-actin to confirm equal loading, and densitometry was performed using ImageJ software (US National Institutes of Health; http://rsb.info.nih.gov/ij/).
After stripping the probes, the same blots were reprobed with U6 probe that serves as a loading control.
After several washes in TBST, the blots were reprobed with a polyclonal actin (I-19) antibody from Santa Cruz Biotechnology, Inc.
The blots were reprobed with antibodies against mouse anti-β actin (1∶5000; Abcam, Cambridge, UK).
The blots were reprobed with anti-ERK antibodies as a loading control.
Blots were reprobed with GAPDH to equalize sample loading.
The Northern blots were reprobed with 5S RNA for gel loading of each Northern blot.
The same blots were reprobed with GAPDH or tubulin, α antibody as a loading control.
Blots were reprobed with anti- α-tubulin antibody (1 : 2000)(Dako) to confirm equal loading of protein.
Blots were reprobed with a mouse monoclonal anti-β-actin antibody (Sigma) to ensure equal loading.
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