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The blots were removed from the manifolds, washed and incubated with goat anti-mouse secondary antibody conjugated to the Alexa680 fluorophore (Molecular Probes).
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Samples were removed from water, blotted, and weighed to obtain swollen mass (Ws).
The Nitrocellulose membranes were removed from the blotting apparatus and dried completely at room temperature.
After 24 h, the fixed aortic arches were removed from formalin, blotted dry, snap frozen and stored at −80°C for plaque analysis.
Spots identified as differentially regulated were removed from the blot and subjected to "in-gel" tryptic digestion as described previously [ 23].
After an interval of 30 min, the hydrogels were removed from the buffer solutions and blotted gently with blotting paper and weighed.
Upon completion of the force generation analysis, the muscles were removed from the baths, blotted dry and weighed.
After depuration for 1, 2, or 3 days, the worms were removed from their containers, blotted dry, then added to biological oxidizer boats with 100 mg d-mannitol.
Strips were removed from equilibration solution, blotted, and placed on a 1-mm thick 25.5 × 20.5 cm 12.5% acrylamide non-gradient SDS gel.
The test compounds were added to the pH 7.4 Krebs-Ringer solution containing 25 mM theophylline and the muscles were incubated for 1 hour after which they were removed from the baths, blotted dry and snap frozen in liquid nitrogen.
Lung tissues were removed from sucrose, excess was blot off, and tissues were placed into molds.
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