The Northern blots were quantitated using ImageQuant v1.2 and the ratios of induced FLO11, relative to ACT1 internal control RNA were thus obtained.
Western blots were quantitated by densitometric analysis.
All western blots were quantitated using ImageJ (National Institutes of Health) densitometry software.
Blots were quantitated using Gel-Pro (MediaCybernetics, Silver Spring, MD).
Where indicated, western blots were quantitated using ImageJ software.
Scanned Western blots were quantitated using MultiGauge Analysis Software (Fujifilm, Tokyo, Japan).
The blots were quantitated using the Biometra BioDocAnalyze 2.0 software (Biometra, Göttingen, Germany).
Blots were quantitated by densitometry and normalized using β-actin to correct for differences in protein loading.
The expression levels of proteins on the western blots were quantitated using densitometer and PD Quest 7.4.0 software (BioRad, Hercules, CA).
However, the Western blots were quantitated on the basis of control lanes loaded according to ATase activity and not total ATase protein and this might not be ideal.
