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Bound antibodies were detected and using an Odyssey fluorescent imaging system (LI-COR Biosciences); blots were quantified according to manufacturers' instructions and normalized to independent actin loading controls.
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(C) Relative protein levels of p62 of individual mice in (1B) were quantified according to the results of ten independent blots and normalized to β-actin.
Total chlorophylls were quantified according to [ 72].
LC3 dot punctae were quantified according to the guideline in detecting autophagy by counting percentage of LC3-positive cell; (b) western blot analysis for LC3II/LC3I ratio and expression of SQSTM1/p62, LAMP2.
The Western blots were quantified and plotted according to the average band intensity of 3 independent experiments, as determined using the ImageJ software.
PVDF membranes were then exposed to photographic film and western blot bands from several different blots were quantified using the NIH Scion Image according to the software guidelines.
All Western blots were quantified by measuring the band density.
Blots were quantified using ImageJ software.
Western blots were quantified using ImageJ [86].
Blots were quantified in ImageJ 1.34s (NIH, USA).
For statistical purposes the blots of three independent experiments were scanned and blots were quantified using NIH Image J program.
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