Blots were processed with Enhanced chemiluminescence (ECL) plus Western blotting detection kit and the signal detected using an LAS-3000 imanalyzeryzer (Fuji Photo Film Co).
Western blotting onto nitrocellulose (0.45 μm, Schleicher and Schuell) was performed according to standard protocols and blots were processed with a chemiluminescence system (ECL, Amersham).
Blots were processed with ECL Plus Western Blotting detection kit and the signal detected using an LAS-3000 imanalyzeryzer (Fuji Photo Film Co., Tokyo, Japan).
The blots were processed with ECL Chemiluminescence kit (Amersham Biosciences), and the signal was detected by exposing the processed blots to X-ray films (Biomax Films, Kodak, NY, USA).
After blotting with respective antibodies, blots were processed with SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL, USA) and exposed to BioBlot BXR film (Laboratory Products Sales , Inc Rochester, NY, USA).
The blots were processed with ECL chemiluminescence kit (Amersham Biosciences, Piscataway, NJ, USA), and the signal was detected by exposing the processed blots to X-ray films (Biomax Films, Kodak, NY, USA).
The proteins were blotted on nitrocellulose and the blot was processed with chemiluminescent detection using the WesternBreeze kit (Invitrogen), following the manufacturer's protocol except that blocking was done overnight at 4°C.
Blots were processed and incubated with antibodies as described above.
Histological images and western blots were processed for quantification with Image Pro Premier software (Media Cybernetics, Rockville, MD).
Briefly, blots were processed as above, but incubated with a fluorescent secondary antibody allowing for detection with the Li-Cor Odyssey infrared system.
All the blots were processed in parallel and were derived from the same experiment.
