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Blots were processed using an ECL kit (Santa Cruz) and exposed to the film.
Blots were processed using an ECL Kit (Santa Cruz) and exposed to X-ray film.
For detection, blots were processed using enhanced chemiluminescence kits (Amersham Pharmacia; Baie d'Urfe, QC, Canada).
Blots were processed using LI-COR Odyssey Fc Imaging system.
Blots were processed using the Brightstar Detection Kit (Ambion) and developed on film.
Blots were processed using an ECL kit and exposed to X-ray film.
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Validation by western blotting was processed using the pooled and individual mitochondrial samples in both PBC mouse model and control groups at different time points.
To determine whether their processing and/or abundance changed as a consequence of loss of Wfs1 in the PFC, Western blots for each of these proteins were processed using brain extracts collected from wt and Wfs1/CKO mice under baseline conditions and following ARS.
Cell lysates and recombinant proteins were processed using SDS-PAGE and Western blotting according to the standard procedures.
Extracts were processed using standard SDS-PAGE and western blotting procedures.
Western blot analyses are processed using the following protocol.
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