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Following SDS-PAGE electrophoresis and transfer, blots were probed using polyclonal anti-ScMre11 (GeneTex) at 1∶2000 and anti-β-actin (Abcam) at 1∶2000.
The blots were probed using secondary antibodies coupled to either IRdye 680 or IRdye 800 (LICOR bioscience, UK) at 1/20000 dilution, and then scanned using an Odyssey Infrared imager (LICOR bioscience, UK). 30 ODs of yeast strains transformed with appropriate plasmids were spheroplasted with zymolyase.
Blots were probed using the primary antibodies.
Blots were probed using corresponding primary antibodies.
Blots were probed using the appropriate antibody for the tagged protein.
The Northern blots were probed using single-stranded internally labeled DNA probes as described previously [ 6].
Similar(41)
After transfer to nitrocellulose, the blot was probed using the monoclonal antibody against FGF8.
The blot was probed using an antibody against rabbit anti-human-profilin-1 (1:1500) and subsequently incubated with FITC-conjugated secondary rat anti-rabbit IgG protected from light.
The blot was probed using a primary antibody against poly (ADP-ribose) polymerase (PARP), caspase-3, bcl-2, bax, cytochrome C, and β-actin (Santa Cruz Biochemicals, Santa Cruz, CA).
The blot was probed using a primary antibody against p-eNOS, total-eNOS (Millipore), Nrf2, HO-1, and β-actin (Santa Cruz Biochemicals, Santa Cruz, CA, USA).
Western-blot membranes were probed using primary antibodies against cathepsin D, p53, PTEN, and α-tubulin (Santa Cruz Biotech, Santa Cruz, CA, USA) and ER α (Ventana Medical Systems, Tucson, AZ, USA).
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