Sentence examples for blots were prepared using from inspiring English sources
In complementary experiments, Western blots were prepared using control tissue obtained from an adult rat-brain microsomal preparation or the total lysate from the SVZ eNPC (Figure 2 C).
Blots were prepared using antibodies as described [23].
Lysates for western blots were prepared using the PARIS™ kit with a protease inhibitor cocktail (Sigma).
Colony blots were prepared using Whatmans 541 filter paper (Whatman, England).
Western blots were prepared using extracts of H. polymorpha and P. chrysogenum cells, obtained using the TCA method [27], and decorated using antibodies raised against IPNS, ACVS, IAT, PCL [8], [28], or the His6 tag (Santa Cruz Biotechnology, INC).
After electro-transfer to a PVDF membrane (pore diameter, 0.45mM), western blots were prepared using antibody 369 (which recognizes the C-terminal region of APP and APP-CTFs) or anti-b-actin (Santa Cruz Biotechnology, sc-4778).
According to the protein concentrations, samples for western blot were prepared using the 4× NuPage LDS sample buffer (Invitrogen , Inc.
Cell lysates for western blot were prepared using SDS-buffer (62.5 mℳ Tris-EDTA, pH6.8, 5% SDS) and total protein content was measured using the BCA protein assay kit (Bio-Rad Laboratories, Hertfordshire, UK).
For the control membrane, a similar blot was prepared using biotinylated soybean trypsin inhibitor (Fluorokine® kit).
Northern blot was prepared using 20 μg total RNA and probes were prepared by labeling the PCR amplicons with αP-dATP using HexaLabel DNA labeling kit (Fermentas Life Sciences, USA).
