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Northern blots were performed with RNAs from both transfections.
Quantitative analyses of blots were performed with Quantity One software (Bio-Rad).
Western blots were performed with Anti-FLAG M2 monoclonal antibody, Sigma #A8592; and Phosphoglycerate Kinase Monoclonal Antibody, Invitrogen #459250.
These samples were dissolved into Laemmli buffer for SDS PAGE and Western blots were performed with primary antibodies.
When necessary, additional blots were performed with serially diluted samples to obtain a signal in a linear range.
All western blots were performed with whole cell lysates, resolved by SDS-PAGE, transferred to PVDF (Immobilon-P, Millipore, Billerica, MA), and blotted for SOCS-1.
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Densitometric analysis for the blots was performed with Bandscan image software.
The densitometric analysis of the Western blots was performed with Image J (Scion).
Densitometric analysis of western blots was performed with the program ImageJ (NIH).
Membranes were developed using ECL chemiluminescence reagent (PerkinElmer) and quantitation of western blots was performed with ImageQuant TL (Amersham Biosciences).
Quantitative analysis of western blots was performed with the NIH Image J software.
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