Western blots were performed using an iBlot2 device (Thermo Scientific) on nitrocellulose membranes.
(B) Using total cell extracts from liver and small intestines (SI), Western blots were performed using antibodies toward human UGT1A1 and mouse CYP2B10.
Western blots were performed using standard protocols.
Western blots were performed using established protocols.
Western blots were performed using the iBlot system (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol.
Western blots were performed using minigels (Bio-Rad) as described previously [42].
All Western blots were performed using the X-BLO T Chamber(www.isenet.it).it
Lysates were immunoprecipitated with antibodies as indicated, and Western blots were performed using streptavidin-HRP (Invitrogen).
All western blots were performed using starting material amounts within the linear range of detection.
To validate our proteomics data, Western blots were performed using available phosphorylation-specific antibodies.
Western blots were performed using the primary antibodies mentioned above, at a 1∶1000 dilution.
