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Western blots were performed on cytoplasmic extracts from renal cells.
Western blots were performed on protein extracts from C2C12 cells.
Western blots were performed on fresh conditioned culture media obtained from E15 dissociated cultured neurons.
Western blots were performed on pooled samples of n = 3 5 pancreata per time point and genotype.
To determine whether L478T/C479T channel proteins were in fact expressed, Western blots were performed on whole-cell lysates.
Westerns Blots were performed on lysates of 1×106 CFU of B. pertussis, or B. bronchiseptica in 10 µL Laemmli sample buffer (Bio-Rad, Hercules, CA).
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Densitometry of bands on Western blots was performed on the Alpha Innotech— AlphaImager 3400.
The qPCR and the western blot were performed on T cells isolated at the same time and each repetition represents one donor.
H2O2 treatment, real-time PCR and western blot were performed on cells 48 h after transfection with the respective siRNA.
Real-time PCR and Western blot were performed on T47D-Hes-6 or mock cells to investigate the transcriptional effects of Hes-6.
Quantification of western-blots was performed on scanned images.
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