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All antibodies for Western blots were obtained from Santa Cruz.
Total P. gingivalis lysate (1×105 mid-late log cells) for Western blots were obtained from cells passaged twice in GAM, twice in GAM-CSE, or twice in GAM-CSE then reconditioned in fresh GAM.
Human multiple-tissue northern blots were obtained from BD Biosciences (Palo Alto, CA, USA), and analysed according to the instructions of the manufacturer.
Parallel DNA blots were obtained from each PCR amplification and hybridized with the telomeric probe (TTAGGG 3, end-labeled with γP-ATP.
Southern blots were obtained from the PCR products and hybridized either with a probe complementary to the pHRT plasmid, as indicated in Figure 4, or with the telomeric probe (TTAGGG 3.
Midveins were dissected and analyzed for LPI 9-11 and LPI 15-17, but insufficient quantities of protein for western blots were obtained from midveins of LPI 3-5.
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Goat anti-ApoB antibody (for immunoflurescence) was from Chemicon International and mouse monoclonal anti-ApoB antibody (1D1, for Western blots) was obtained from the Heart Institute of the University of Ottawa (Canada).
All chemicals for lectin blotting were obtained from Sigma.
Antibodies for Bax, p53 and Tyrosinase for western blotting were obtained from Santa Cruz Biotechnology Santaa Cruz, CA, USA).
Peroxidase-conjugated secondary antibodies for Western blotting were obtained from Sigma Aldrich.
Secondary horseradish-peroxidase-conjugated antibodies for Western blotting were obtained from Jackson Immunoresearch Laboratories.
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