Sentence examples for blots were next washed from inspiring English sources

The blots were next washed in PBS with 0.5% Tween 20 and re-probed with mouse anti-actin serum (1 100; JLA20, Developmental Studies Hybridoma Bank, the University of Iowa, USA).

Cochleas were next washed with blocking solution for 4 h.

Cells were next washed twice in a wash buffer for 5 min at room temperature.

The next days, blots were washed three times for five minutes each with PBST, incubated with HRP-conjugated goat anti-mouse or anti-rabbit secondary antibody (Pierce), washed again, and exposed with ECL reagent (Amersham).

Secondary antibody was then applied and allowed to incubate for 10 min before blots were again washed three times with PBST.

Secondary anti-rabbit HRP (GE Healthcare) was used at 1 5000 dilution for 1 h at RT and the blots were then washed as above.

The blots were then washed in 1x TBST four times for 10 min.

The blots were then washed 3× for 10 min each time, with TBS-T.

Blots were then washed three times at 50°C and autoradiographed.

The blots were again washed in TBS-T.

Blots were stripped, washed, and reprobed for β-actin.