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Membranes were analyzed with enhanced chemiluminescence Plus reagents (GE Healthcare, #RPN5781) and scanned with a Storm Scanner (Amersham Pharmacia Biotech Inc .. Integrated densities of the bands in the western blots were measured using Image J software (NIH).
Intensities of protein bands on blots were measured using the Bio-Rad ChemiDoc densitometer.
For densitometric analyses, the bands on the blots were measured using the Eagle Eye II imaging system.
For densitometric analyses, the bands on the blots were measured using an Eagle Eye II imaging system.
Then, the gray intensities of blots were measured using ImageJ software (Bethesda, MD, USA) and were normalized for β-actin or B23.
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The intensity of the blots was measured using Image J (version 1.32; National Institutes of Health, USA) software.
The densitometries of Western blots are measured using ImageJ v1.48 and indicated below the blots as a ratio of band intensity normalized to the loading control β-actin or the protein used for IP.
The relative intensity of the protein on Western blot was measured using AlphaEaseFC Analysis tools (Alpha Innotech).
Band intensities in northern blot analyses were measured using a Bio-Rad Discovery Series Quantity One® image analyzer and software (Bio-Rad,Hercules,CA).
For quantitation of Western blots, ECL signal intensities were measured using a Storm 860 Imaging System (GE Healthcare, Piscataway, NJ).
The supernatant was used for Western blot and caspase activities were measured using a colorimetric assay system following the protocol described by the manufacturer (Promega for caspase-3 [G7220], Millipore for caspases 8 [and171] and 9 [APT173]).
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