Sentence examples for blots were made from from inspiring English sources

Quantitative spot blots were made from batch samples, from clones seeded at 5 × 105 cells/ml and grown for 3 days (CHO-SF) or 5 days (293SF) under identical conditions at 30°C.

Therefore, Western blots were made of proteins extracted from late-stage embryos (Figure 4A and B).

Southern blots were made using 20 µg DNA and the Vaku-Blot system (Biometra).

"Virtual northern blots" were made using the Clontech SMART cDNA Synthesis Kit, according to the manufacturer's instructions using RNA from the stages indicated.

In two experiments, western blotting samples were made from pooled adherent and floating cells.

Protein extracts for western blot analysis were made from whole flies, sampled after 7 days of food treatment, using a TCA-based extraction protocol.

For western blot analysis, total lysates were made from the spreading cells.

The quantification of the intensity of the bands from western blots was made using Image J. The values included in the graph represent the average from 3 experiments after the normalization; in each case, we used pools of material from 4 animals per genotype and the data represent mean ± SEM.

After 48 hours of culture, nuclear extracts were made from all cultures, western blotted for NF-κB p52 and quantified.

To quantitate the GFP bands from the Western blot, densitograms were made using ImageJ software (http://imagej.nih.gov/ij/, last accessed April 30 , 2015.

Protein extracts were made by TCA extraction and analysed by western blotting as previous described58.