Sentence examples for blots were hybridized using from inspiring English sources
The blots were hybridized using ULTRAhyb hybridization buffer (Applied Biosystems, Waltham, MA, United States) according to the manufacturer's instructions.
Dot blots were hybridized using standard procedures detailed elsewhere [47].
Mouse adult multiple tissue and embryonic Northern blots were hybridized using a probe generated from mouse exon 2 (probe 1, Fig. 1B), yielding a band of approximately 2.4 kb (isoform 1), as expected, and a less abundant and unexpected 4.4 kb band (isoform 2) (Figs. 3A & 3B).
The blot was hybridized using a standard protocol with 1 × 106 cpm probe per ml of hybridization solution [ 10].
The Northern blot results were quantified using signals obtained from a serial dilution of equivalent in vitro transcripts, which were hybridized using the appropriate probes in parallel.
Metaphase preparations were hybridized using a 21Xmouse mFISH kit (MetaSystems).
A commercial human multiple-tissue northern blot (Clontech) was hybridized using a [P]-labeled 400 bp NotI-GPR99 fragment of the IMAGE clone 3014233 (according according to the manufacturer's instructions.
Labeled cRNA was hybridized using Gene Expression Hybridization Kit (Agilent).
The blots were hybridized with radioactive probes using standard techniques.
Blots were hybridized overnight, and bands were visualized using a Phosphorimager (Molecular Dynamics).
Blots were hybridized with 5' 32P-labeled oligodeoxynucleotides (Table 3) using RapidHyb Buffer (Amersham).
