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Western blots were generated with protein extracts from XIAP injected eyes and contralateral uninjected control eyes.
Density blots were generated with R studio.
Third, DNA gel blots (i.e., Southern blots) were generated with total insect DNA and hybridized with probes located adjacent to repeats in the Portiera BT genome.
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Kaplan Meier blots were generated to evaluate links with patient survival and the log rank test used to compare curves separated according to MSX2 expression.
Western blots were generated from 2-D gels of both bovine pericardium protein fractions.
Western blots were generated using standard procedures.
Graphs were generated with Microsoft Excel program.
The lysates for western blotting were generated using standard 1XRIPA buffer supplemented with 1X Protease Inhibitor Cocktail (Sigma).
Crude cell extracts for western blotting were generated as described (Knop et al. 1999).
The luminescent signal was generated with the ECL Western blotting detection reagent kit (Amersham Pharmacia Biotech, Buckinghamshire, UK), and the blots were exposed to medical X-ray film (Curix RP1; Agfa Argentina, Florencio Varela, Buenos Aires) for 10 s to 5 min. Band intensity was not quantified if the film was saturated.
Pictures have been generated with MacPyMOL.
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blots were treated with
blots were reacted with
blots were visualized with
blots were revealed with
blots were incubated with
blots were processed with
blots were hybridized with
blots were analyzed with
blots were stained with
blots were reprobed with
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blots were performed with
blots were blocked with
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