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Western blots were generated from 2-D gels of both bovine pericardium protein fractions.
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To directly compare the distinct species of ubiquitinated protein (-Ub1, -Ub2, -Ub3, -Ubn), lane profiles of the western blots were generated for each reaction.
Two blots were generated in parallel from 10 µg per well of total RNA extracted from single MatDp(dist2), PatDp(dist2) or non-UpDp control newborn brains.
Western blots were generated with protein extracts from XIAP injected eyes and contralateral uninjected control eyes.
Western blots were generated using standard procedures.
Density blots were generated with R studio.
Densitometry scans were generated from the Western blots and the specific bands are shown in Figure 3b.
Control and experimental groups were generated from the same blot.
Membrane blots are generated by fixation of proteins upon growing E. coli clones on membranes and microarrays are spotted using proteins from distinct 1 ml culturing of clones.
Since Southern blot analysis suggested the presence of two to three IFN genes, additional cDNAs were generated from catfish fibroblast and lymphoid cell lines using primers designed to conserved regions of zebrafish and catfish interferon.
Embryos were generated from natural crosses.
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