Sentence examples for blots were further washed from inspiring English sources

The blots were further washed with TBST, and specific proteins were visualised by using enhanced chemiluminescence western blotting detection reagents (GE Healthcare).

The cells were further washed twice.

The washed pretreated bagasse was further washed with 50 mM NaOH solution (2 × 20 mL/wash) followed by further water wash (2 × 100 mL/wash).

After washing, blots were further incubated with 1 2,000 diluted secondary antibodies including horseradish peroxidase (HRP -conjugated goat anti-mouse, goat anti-rabbit, or rabbit anti-goat IgG for 2 HRP -conjugatedrature.

Following washing, the blots were further probed with horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit Igs and developed using an enhanced chemiluminescence system (GE Healthcare).

Subsequently, the blots were further incubated with the secondary horse radish peroxidase (HRP -conjugated anti-rabbit antibody for 30 min. After wasHRP -conjugatede visualized using the ECL™ Western blotting detection system (Amersham).

(d) Western blot were further confirmed the expression of FoxO3a, SOD2 and CAT.

Blots were again washed in PBST and further incubated with HRP-conjugated secondary antibody (Santa Cruz Biotech USA).

Blots were then washed in PBS and further incubated with secondary antibodies, Dylight 680 anti-mouse (1 5000 v/v) and Dylight 800 anti-rabbit (1 5000 v/v), for 1 hour at room temperature.

The blots were then washed in 1x TBST four times for 10 min.

The blots were then washed 3× for 10 min each time, with TBS-T.