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Blots were finally washed in several changes of PBS and rinsed in water to remove excess stain and detergent before excision and spot-analysis by mass spectrometry.
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The cells were finally washed and analyzed in FACS Calibur.
The stained cells were finally washed with water.
The wells were finally washed three times.
Acceptance is finally washing those plates.
Blots were finally exposed to Hyperfilm MP (Amersham, GE Healthcare, Madrid, Spain).
The blot was finally air dried and exposed for autoradiography.
The blot was finally exposed to Hyperfilm MP (Amersham, GE Healthcare).
Blots were washed finally at 50°C, 1 × SSC, 0.1% SDS and visualized by autoradiography.
Finally, blots were washed for 3 × 5 min in washing buffer, developed using the enhanced chemiluminescence kit (ECLplus, GE Healthcare), and visualized on Kodak film.
Finally, the blots were washed again three times with immune-blot washing buffer before detection of light emission with the BM chemiluminescence immune blotting kit (Roche Applied Science) as described by the supplier.
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