The relation between expressions measured with QPCR and western blot was evaluated with bivariate correlation using Spearman correlation coefficient.
Western blots were evaluated using AIDA software (Raytest GmbH).
Blots were evaluated by chemiluminescence imaging (Las 1000, Fuji, Japan).
In a dot blot assay, individual yBRDs binding to a complete set of the histone peptides spotted on the membrane were evaluated with Western blotting using anti-GST antibody (Figure 2A).
iNOS and Arg1 were used as M1 and M2 markers, respectively, the results were evaluated with Western blotting.
Cytoplasmic and nuclear proteins which were extracted from three immortalized cell lines (HEK293, HaCaT, GES-1) and ten cancer cell lines (cervical cancer cells C33A, CaSki, Hela, and SiHa; gastric cancer cells AGS, SNU-1, N87, SNU-16, BGC823 and MGC803) were evaluated with Western blot.
Expression of 14-3-3σ 14-3-3σ 14-3-3σn were evaluated with real-timRNAT-PCR and Western Blot analysis, resproteinly.
The data from in situ hybridization or Western blotting were evaluated by one-way ANOVA with intergroup differences analysed by Fisher's Protected Last Significant Difference PLSD test, corrected by Bonferoni's procedure for dependent samples.
The values of each target blot were evaluated.
To determine whether 1A6/DRIM functions in Pol I transcription, 47S rRNA level was evaluated with Northern blotting when 1A6/DRIM was silenced by siRNA.
