Equal loading of the protein in each group on the blots was evaluated using anti β-actin anti β-actiner using Restore WB stripping buffer (Thermo Fisher Scientific, Bantibodyn, IL).
IS molecules were evaluated using Western blot analysis followed by computer-assisted densitometer evaluation.
Bax, bcl-2, caspase-9 and activated caspase-3 protein expression were evaluated using western blot analysis on nitrocellulose membranes prepared as reported for ALR western blot analysis.
Apoptosis and TRPV1 were evaluated using Western blot analysis.
In this study, the acute effects of recovery between mild head injuries and the frequency of injuries on a headache behavior, trigeminal allodynia, was assessed using von Frey testing up to one week after injury, while histopathological changes in the trigeminal pain pathway were evaluated using western blot, ELISA and immunohistochemistry. .
Insulin receptor substrates (IRS-1, IRS-2) and post-receptor signaling molecules Akt, mammalian target of rapamycin (mTOR), ribosomal p70s6 kinase (p70s6k) and the eukaryotic translation initiation factor 4E (eIF4E -binding proteIF4E -binding as well as the aproteinc marker caspase-3 were evaluated using Western blot analysis.
Levels of these proteins were evaluated using western blot analysis.
At the end of each time point, pERK1/2 protein levels were evaluated using western blot analysis.
While the MMP-3 and MMP-13 protein expressions were evaluated using western blot method.
