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Blots were evaluated by chemiluminescence imaging (Las 1000, Fuji, Japan).
After additional PBST washes, the blots were evaluated by using an enhanced chemiluminescent system.
Signals from developed Western blots were evaluated by scanning densitometry using ImageJ software, and results were determined as background-corrected absorbance units.
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Brevican density from human tissue on Western blots was evaluated by one way ANOVA followed by Bonferroni multiple comparison test.
The data from in situ hybridization or Western blotting were evaluated by one-way ANOVA with intergroup differences analysed by Fisher's Protected Last Significant Difference PLSD test, corrected by Bonferoni's procedure for dependent samples.
Blot-protocols were evaluated by manual measurements of blot signal density.
Hybridisation signals of both, MTN blots and the CPA, were evaluated by use of a STORM-860 phosphoimager (Molecular Dynamics, now part of GE Healthcare).
The protein levels were analyzed by Western blot and the mRNA levels were evaluated by real-time PCR.
For this, EAG1 protein levels were evaluated by Western blots of proteins extracted from tumors derived from T-47D cells.
The antigenicity and reactionogenicity of the rCMEPX were evaluated by western blot and indirect ELISA.
The expressions of Hsp72, p65 and I-kappa B were evaluated by Western blot and immunochemical analysis.
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