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After incubation with second antibody (1 : 2500) for 1 h at room temperature, the blots were determined using an ECL Western blotting kit (Amersham Pharmacia Biotech, Buckinghamshire, UK), according to the manufacturer's instructions.
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The intensity of the bands in stained gels and blots was determined using Nonlinear.
Density of bands on film exposed to blots was determined using Scion Image software (Scion Corp., Frederick, MD, USA).
Immunodetection of the protein blotted was determined using a Fujifilm Intelligent Dark Box II.
Relative band density for Western blotting was determined using ImageJ gel analysis software.
Intensity of the blot was determined using the Image J software.
Intensity of the blot was determined using the Image J software (NIH, Bethesda, MD, USA).
Statistical significance in glucose uptake assays, glycogen synthesis assays, and Western blotting was determined using RM two-way ANOVA followed by the Bonferroni multiple comparison test.
The COX2, prostaglandin E2 receptor 4 (EP4), and leukotrienes B4 receptor (LTB4R) antibody blot densities were determined using the same process.
For Western blotting, protein concentrations were determined using a Bradford reagent (BioRad).
The western blot band densities were determined using AlphaEase FC Software AlphaInnotech , Inc).
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