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Data from western blots were compared using a two-way ANOVA followed by Tukey or Sidak post-hoc comparisons.
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For quantification, the density of bands from three blots was compared using Image J. 12-week-old male Sema3AKD mice and their littermates not expressing Cre (as controls), were used for surgery as above, with five mice for each group and time point.
The image data from western blotting was compared using one-way ANOVA.
The relative intensities of the Western blot membranes were compared using Alpha Ease software.
Receptor levels in the stable clones were compared using western blot analysis.
First, tissues from different anatomical layers of ST36 and the sham point were sampled, and channel expressions between the two points were compared using western blotting.
Expressions of the klotho protein in the medulla oblongata between Wistar rats and STZ-diabetic rats were compared using Western blotting analysis.
Forty-five clinical samples of breast tissues including normal breast, ductal carcinomas in situ, invasive ductal carcinomas, and their metastases to the brain were compared using Western blot analysis and immunohistochemistry for various chains of laminin, in particular laminin-8 and laminin-9.
The amounts of pCofilin and Cofilin in aliquot 1 and aliquot 2 were compared using following Western blot analysis.
All Western blot experiments were conducted in triplicate and results were compared using the Student's t-test, p<0.05.
Twenty micrograms of total protein, as determined using Bradford assay, were compared using the SDS PAGE and western blot analysis.
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