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Northern blots were analyzed using BAS 3000 (FujiFilm, Tokyo, Japan).

Total proteins were separated by SDS gel electrophoresis, and western blots were analyzed using the antiserum to TuMV CP.

The blots were analyzed using the LICOR Odyssey Infrared Imaging System, and the signals of the target proteins were normalized to the signal of β-actin which acts as a house-keeping protein.

Blots were analyzed using an Odyssey imaging system.

After extensive washing, the blots were analyzed using the LI-COR Odyssey infrared imaging system (Biosciences).

Northern blots were analyzed using a FLA3000 (FUJI) or a LAS Reader 3000 (FUJI).

The blots were visualized using Immobilon Western (Millipore), and the intensity of the blots was analyzed using a Gel-Pro Analyzer version 4.0 (Media Cybernetics, Bethesda, MD, USA).

The images taken were assembled using Adobe Photoshop and band intensity on the Western blots was analyzed using ImageJ.

An image of the Ponceau membrane and each blot were analyzed using the ImageJ software (NIH).

All bands from Western blot were analyzed using Image J software (version 1.6 NIH) to verify the relative levels of Notch signaling and cardiomyocyte-specific markers compared to β-actin.

Western blot data were analyzed using unpaired Student's t-test.