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The obtained blots were analyzed by densitometry using the software Gel-Pro analyzer v. 3.1.

Western blots were analyzed by densitometer scanning using Alphaview software (Alpha Innotech, Santa Clara, CA).

Blots were analyzed by chemiluminescence using either the ECL Kit (GE Life sciences, Piscataway, NJ) or the Hyglo Kit (Denville Scientific Inc., Metuchen, NJ).

Time series of serum measurements and results from Western blots were analyzed by ANOVA for repeated measurements or by using area under curve (AUC).

The blots were analyzed by chemiluminescence detection.

Blots were analyzed by UN-Scan IT software.

Band intensity (area × density) on Western blots was analyzed by densitometry (model GS-700, Imaging Densitometer; Bio-Rad, Hercules, CA).

Data from western blotting were analyzed by paired t-tests using SAS version 12.6.

The densitometry quantification of dot-blot was analyzed by ImageProPlus 6.0.

Each blot was analyzed by comparing the size of the hybridizing fragments with that predicted by the known sequence (Fig. 1A, B).

The correlation between NPM1 mRNA and protein expression by Western blot was analyzed by Pearson's test.