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The blots were analysed using a PhosphorImager (GE Healthcare).
Western blots were analysed using image densitometry with TotalLab TL100 software (Nonlinear Dynamics, Durhan, USA).
Blots were analysed using Image J software to semi quantify individual bands.
Fluorescent intensity of bands on western blot and dots on dot blots were analysed using ImageQuant software.
Western blots were analysed using HRP-conjugated secondary antisera (Sigma), or using the Odyssey infrared system (Licor, Lincoln, NE, USA).
The developed blots were analysed using the Image J program to quantitate the intensity of the bands and the values for Mpl expression are shown in Fig. 11.
Similar(51)
At each probing, the blot was analysed using a PhosphorImager.
Blot scans were analysed using the Odyssey software to manually define bands.
To confirm the deep sequencing results, the expression levels of miR156a, miR157 and miR164a were analysed using northern blot.
The blots were imaged and the density was analysed using FluorChem®SP imaging software (Cell Biosciences).
Bound proteins were analysed by western blotting using anti-GST (GE Healthcare) and anti-Hog1 (yC-20, Santa Cruz Biotechnology) antibodies.
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blots were scanned using
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