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PAR western blots were also used to evaluate the time course of PAR formation.
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The same antibodies that were employed for western blotting were also used for ICH.
Similar attempt to detect native recombinant Crym protein using the affinity purified IgY chicken anti-Crym (Michel see below for western blot) were also used unsuccessfully.
qRT-PCR and western blotting were also used to confirm the effect of miR-375 in inhibiting IGF1R at both mRNA and protein levels.
Reverse transcription polymerase chain reaction and Western blot were also used to analyse the Six1 mRNA and protein expression patterns in normal liver tissues.
Genomic DNA blotting was also used to evaluate CDKN2 gene deletions.
Western blotting was also used to measure tissue OPN (antibody developed by J.C. at Pfizer) and RBP4 (Adipogen, Inc .. Signal intensities of chemiluminescence autoradiographs were densitometrically quantified using a digital Kodak 3D Imagestation and associated digital image analysis software (Kodak,).
Southern blotting was also used to verify the presence of α1 and α3 genes.
Western blotting was also used to detect a reduction in pFLT3 expression after 300 mg and 525 mg twice-daily dosing with MLN518/tandutinib.
Finally, Northern blotting was also used to confirm the Bombyx 5.8 s rRNA-derived small RNAs, demonstrating that various novel small RNAs exist in the silkworm.
The extent of cleavage of poly(ADP-ribose) polymerase (PARP) -1 and caspases 3 and 8, determined by western blotting, was also used as an indicator of apoptosis.
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