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The blots were also stripped and re-probed with non-radioactively labeled DNAs corresponding to HygR DNA released from pHP45ΩHyg [30] by HindIII digestion or AprR DNA released from pIJ773 [31] by EcoRI and HindIII digestion.
Similar(59)
Membranes were also stripped with the Restore Western Blot Stripping Buffer (Thermo Fisher Scientific Inc).
They were also stripped of their property.
He was also stripped of those victories.
For successive antibody probes, blots were stripped using Pierce Restore™ stripping reagent with modifications to the manufacturer's protocol.
Before reprobing blots were stripped with Restore WB stripping buffer from Pierce.
Western blots were stripped between hybridizations with stripping buffer [10 mM Tris HCl (pH 2.3) and 150 mM NaCl].
Blots were then stripped using Restore western stripping buffer (Pierce) and equal loading of samples verified by β-actin staining.
The blots were then stripped and blotted for beta-tubulin (55 kDa) to assess equal gel loading.
The blots were subsequently stripped and reprobed with tubulin antibody.
The blots were then stripped by incubation in stripping buffer [ 25] and reprobed a loading control.
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