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Total phospho-JNK levels of the same blots were also determined to quantify the levels of phospho-JNK2 isoforms, in a similar manner as for the JNK1 isoforms.
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Apoptotic cell death (DNA fragmentation, nucleus condensation and cytokeratin 18 cleavage) and inhibition of the mevalonate pathway (western blot) were also determined.
To verify that JNK was not stimulated in this treatment condition, input p-JNK loaded on the same western blot was also determined as positive control.
Endothelins (ETs) and eNOS evaluation (Western blot and immunostaining) were also determined.
The specificity and reactivity of Cel7A and Cel6A PAbs were also determined by Western Blots by using purified Cel7A and Cel6A from Celluclast as well as 2 commercial enzyme mixtures.
Protein and mRNA levels of nuclear factor kappa B p65 (NF-κB p65), intercellular adhesion molecule-1 (ICAM-1) were also determined using western blot and real-time PCR respectively.
Beclin 1 and Atg 7 expression were also determined by Western blot.
Similar results were also determined by western blot analysis in protein extracted from the tumor tissue.
The antibody epitopes were also determined by western blot analysis using bacterially expressed GST-fused insulin A chain, B chain and B chain truncation mutants (1-20 and 1-13).
Phosphorylation of EGFR of cells grown in the serum-free and serum-rich medium at basal levels and following 10 min stimulation with 10 ng ml−1 EGF were also determined by Western blotting, using the Phospho-EGF Receptor (Tyr992) polyclonal antibody (Cell Signalling Technology, USA).
Protein levels of PDF were also determined in 2 colon cancer patients via western blotting.
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