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All ELISA, RT-PCR, HPLC and Western blots were also analysed by two-way ANOVA or Students t-test.
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Cell lines HCT15 and OVCAR8, which showed an altered sized P300 protein on Western blot were also analysed by PTT.
Cell extracts prepared for the ADP-ribosylation assays were also analysed by Western blot.
Sample extracts were also analysed by Western Blot using the anti-GAD65/67 anti-GAD65/67
Cell lysates were also analysed by Western blotting.
Blots were also probed for actinin (anti-actinin, Sigma).
Blots were also probed with U6 probes to normalize loading.
Blots were also revealed with anti-gammaTubulin monoclonal antibody (Sigma) as a loading control.
PAR western blots were also used to evaluate the time course of PAR formation.
Western blots were also reprobed with an antibody recognizing actin (1∶50,000; Sigma-Aldrich) to illustrate equivalent protein loading.
The biotinylation blots were also probed simultaneously with GluR1 and GluR2 antibodies using the ECL plex system, and subsequently reprobed for actin.
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