Sentence examples for blots were again stripped from inspiring English sources

Following detection, blots were again stripped and reprobed for 1.5 h at RT with the panspecific mouse antiactin mAb (clone JLA20) (Oncogene), diluted 1/5000 in 5% blocking solution B, to control for equal loading of protein.

The blot was again imaged.

For successive antibody probes, blots were stripped using Pierce Restore™ stripping reagent with modifications to the manufacturer's protocol.

Before reprobing blots were stripped with Restore WB stripping buffer from Pierce.

Western blots were stripped between hybridizations with stripping buffer [10 mM Tris HCl (pH 2.3) and 150 mM NaCl].

Finally, all blots were stripped and blocked again for incubation with monoclonal antibodies against ERK1/2 (1:2000, Cell Signaling, Beverly, MA).

As loading control, blots were stripped and incubated again with an anti-beta-actin antibody-HRP (Abcam, Cambridge, UK).

Blots were stripped by incubation in stripping buffer (62.5 mM Tris, pH 6.7, 100 mM β-mercaptoethanol and 2% SDS) for 30 minutes at 50°C, blocked again in TBST buffer containing 5% non-fat milk and then probed a second time with polyclonal anti-actin.

Blots were then stripped using Restore western stripping buffer (Pierce) and equal loading of samples verified by β-actin staining.

The blots were then stripped and blotted for beta-tubulin (55 kDa) to assess equal gel loading.

The blots were subsequently stripped and reprobed with tubulin antibody.