By Western blotting, we determined that both ΔC1q and ΔC6 sera could not support the constitutive expression of WOX2 (an isoform of WOX1) and p-ERK in DU145 cells (Figs. 8A and 8B), suggesting that serum C1q and C6 components are essential for the expression of these proteins.
Through Western blot analysis, we determined that all three transgenes had comparable expression levels, thus allowing us to perform direct comparative analyses (supplementary material Fig. S1).
Using the UAS-GAL4 system, we generated transgenic flies expressing the full-length ref(2 P, and after confirming the transgene expression using western blot analysis, we determined that ref(2 P expression reverts the male sterility observed in ref(2 P od3 mutants.
By Western blot analysis, we determined that TDP-43 protein levels in siRNA-treated cells were downregulated to about 10% of the control levels (Supporting Information Fig S2B), but the percentage of downregulation varied among cells (Supporting Information Fig S2A).
Using Western blot analysis, we determined that steroid receptor coactivators (SRCs) 1 3, Akt, phosphorylated Akt, progesterone receptor A (PR-A), and erbB3 proteins were significantly up-regulated at 50 days of age.
By densitometry analysis of the western blot in Figure 2A, we determined that the total incorporation of lipoate into proteins in cells expressing Lpa is 1% of that in cells expressing Lpa S259A.
Western blot analysis determined that during 8 24 h treatment period, similar amounts of p53 expression, elevating to approximately 10 times the original level, were exhibited (data not shown).
Based on Western blot, we have determined that H3 cleavage interferes with the measurement of certain histone modifications.
Using western blot analysis, we further determined that MCF7-TFF3 cexhibitedbitedecreaseded protein expression of CDH1, OCLN, and CTNNG; and higher protein levels of CDH2, VIM, and ITGA6 compared to MCF7-vector cells.
"We can't determine that.
