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An anti-dSETDB1 polyclonal antibody (1∶50 for immunostaining; 1∶5,000 for protein blots) was raised using a synthetic peptide, N-YFDGTTCSRGKDKGC-C, as an immunogen.
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This difference in micronuclei after damage was confirmed by Western blot analysis using an antibody that was raised against an epitope of 53BP1 that is conserved between mice and humans.
SMAR1 polyclonal antibody that was raised in house [ 41] was used for probing the blot.
Antibodies against purified SepF were raised and used to perform a quantitative western blot analysis.
Mpl antibody that was raised against the full length Mpl protein was used for western blot analysis.
Antiserum was raised in rabbits, and antibody specificity was confirmed by Western blotting.
Analysis of blots was performed using ImageJ (National Institutes for Health).
Therefore, to test the specificity of the western blotting, westerns blots were performed using antibodies raised against all three proteins to confirm the exact identity of the protein band.
Peptide-specific antibodies were raised in rabbits and used for Western-blot analysis and for immunocytochemical studies.
Western blots were developed using rabbit antiserum raised against a bacterially expressed fragment of the c-Myb DNA binding domain [ 28].
To validate the effects of the T. indica fruit pulp extract on HepG2 proteins, Western blotting was performed using antisera raised against the cellular proteins.
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