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Signal intensity of Western blots was quantitated using a PhosphoImager (Kodak, Rochester, NY, USA).
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The Northern blots were quantitated using ImageQuant v1.2 and the ratios of induced FLO11, relative to ACT1 internal control RNA were thus obtained.
All western blots were quantitated using ImageJ (National Institutes of Health) densitometry software.
Blots were quantitated using Gel-Pro (MediaCybernetics, Silver Spring, MD).
Where indicated, western blots were quantitated using ImageJ software.
Scanned Western blots were quantitated using MultiGauge Analysis Software (Fujifilm, Tokyo, Japan).
The blots were quantitated using the Biometra BioDocAnalyze 2.0 software (Biometra, Göttingen, Germany).
The expression levels of proteins on the western blots were quantitated using densitometer and PD Quest 7.4.0 software (BioRad, Hercules, CA).
The western blots were quantitated using a National Institutes of Health image analyzer (ImageJ 1.27z; National Institute of Health, Bethesda, MD, USA) and are presented as the ratio of phospho-Akt to Akt or of phospho-eNOS to eNOS (relative phosphorylation) in arbitrary units.
The MIP-2 mRNA, EMSA, and Western blots were quantitated using an NIH image analyzer ImageJ 1.27z (NIH) and presented as the ratio of phospho-MAPK to MAPK (relative phosphorylation) in arbitrary units.
The western blots were quantitated using a National Institutes of Health image analyzer (Image J 1.27z; National Institute of Health, Bethesda, MD, USA) and are presented as the ratio of phospho-MAPK to MAPK or the ratio of phospho-Akt to Akt (relative phosphorylation) in arbitrary units.
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