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The intensity of the blots was measured using Image J (version 1.32; National Institutes of Health, USA) software.
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Membranes were analyzed with enhanced chemiluminescence Plus reagents (GE Healthcare, #RPN5781) and scanned with a Storm Scanner (Amersham Pharmacia Biotech Inc .. Integrated densities of the bands in the western blots were measured using Image J software (NIH).
Intensities of protein bands on blots were measured using the Bio-Rad ChemiDoc densitometer.
For densitometric analyses, the bands on the blots were measured using an Eagle Eye II imaging system.
For densitometric analyses, the bands on the blots were measured using the Eagle Eye II imaging system.
Then, the gray intensities of blots were measured using ImageJ software (Bethesda, MD, USA) and were normalized for β-actin or B23.
The densitometries of Western blots are measured using ImageJ v1.48 and indicated below the blots as a ratio of band intensity normalized to the loading control β-actin or the protein used for IP.
The relative intensity of the protein on Western blot was measured using AlphaEaseFC Analysis tools (Alpha Innotech).
The optical density of the corresponding Western blot band was measured using Kodak ID Image software.
The relative intensity of each protein blotting analysis was measured using a computerized software program (Bio-Rad, CA, USA), and the expression of activated and total VEGFR-2 were normalized with total VEGFR-2 and β-actin bands, respectively, to compare the expression of proteins in different treatment groups.
mRNA expression was analyzed by real-time RT-PCR, protein expression was examined by Western blotting and IKK activity was measured using an in vitro kinase assay.
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