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Equal loading of the protein in each group on the blots was evaluated using anti β-actin anti β-actiner using Restore WB stripping buffer (Thermo Fisher Scientific, Bantibodyn, IL).
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Western blots were evaluated using AIDA software (Raytest GmbH).
Blots were evaluated using a Fluor-STM MultiImager (Bio-Rad Laboratories, HerCAles, CA, USA) and quantified by the quantity one quantitation software (Bio-Rad).
Western blots were imaged and pixel density was evaluated using FluorChem FC2 software (Alpha Inotech; San Leandro, CA).
PR expression was evaluated using Western blots, as described below.
Expression of AMPK, α1, α2, ACC, Akt, the Glut-4 translocation mediator Akt substrate of 160KD (AS160), mTOR, total and membrane Glut-4 was evaluated using Western blot.
Cells underwent I/R, and HSP 72 content was evaluated using Western blotting.
The role of MAPK signaling in calycosin-induced angiogenesis was evaluated using western blotting.
The level of expression of DCC, UNC5H in the VTA of PND23, PND35 and PND90 rats and in the midbrain of E17 and PND0 rats was evaluated using Western blot analysis as described previously [17] [17].
MAPK phosphorylation was evaluated using Western blotting.
Protein expression was evaluated using western blotting.
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