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Densitometry of Western blots was estimated using ImageJ software.
Densitometry of Western blots was estimated using ImageJ software (NIH).
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The integral optical density (IOD) of the Western blot band was estimated using ImageJ.
The expression of gene encoding pro-α1 chain of type I collagen was estimated using Dot-Blot hybridisation technique.
In addition, repeat length was estimated using Southern blotting techniques as previously described [ 35] (Table 1).
The concentration of Nox4 in cell lysates was estimated using Western blotting and allowed calculation of a turnover of ∼200 mol of H2O2 mol−1 (mof of Nox4)−1.
The expression of endoplasmic reticulum stress markers was estimated using western blot analysis, and the results showed that compared to wild-type motor neurons, there was an increase in the expression of BiP, ATF4 and CHOP in AR100 motor neurons (P < 0.05, t-test, for all markers, wild-type versus AR100).
1/S' was estimated using BlastPhen.
RNA concentration was estimated using nanodrop spectrophotometer.
Protein concentration was estimated using Coomassie Plus Protein Reagent.
Nucleotide diversity was estimated using DnaSp 4.10 [38].
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