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Densitometry of blots was done using quantity 1D analysis software and gel doc imaging system.
Densitometric analysis of Western blots was done using the Kodak Gel Logic system.
Quantification of western blots was done using Multi-Gauge (Fuji-Film) and Image J (NIH).
Densitometric analysis of western blots was done using AlphaEase FC software (Alpha Innotech, Santa Clara, CA).
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Quantitative Western Blots were done using the Odyssey Infrared Imagines System (LI-COR Biosciences) using Alexa Fluor680 secondary antibodies (Molecular Probes).
Densitometric analyses of Western blots were done using ImageJ software.
Western blots were done using the mammary tumors and MCF-7 cells treated with hormones.
So half the blots were done using one type or the other (no obvious difference was noted).
Northern blotting was done using standard techniques.
Northern blotting was done using NorthernMax formaldehyde-based system (Ambion Inc., Austin, TX) according to the manufacturer's recommendations.
Final imaging of the blot was done using the Fluor Chem Q system (Protein Simple, Santa Clara, CA).
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