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Densitometry of Western blots was conducted using Adobe Photoshop (Adobe Systems Incorporated, San Jose, CA, USA).
Quantification of the blots was conducted using Image-Pro Plusoftwarere (version 6.0; Media Cybernetics, Bethesda, MD, USA).
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Western blots were conducted using standard techniques.
Western blots were conducted using Rat anti Ndel1 and mouse anti GFP (Sigma-Aldrich, St . Louis MO).
Quantitative densitometric analyses of western blots were conducted using ImageJ 1.44 or ImageQuant.
Protein (50 µg) was loaded onto 10% SDS-PAGE gels and western blotting was conducted using a primary antibody against ADMR (cat # LS-A4048 MBL International Corporation, Woburn, MA) at a 1: 1000 dilution and using a primary antibody against CRLR (cat # sc-30028, Santa Cruz Biotechnology, Santa Cruz, CA) at a 1: 100 dilution.
Western blotting was conducted using routine protocol.
Western blotting was conducted using standard protocols.
Western blotting was conducted using PVDF membrane (GE Healthcare, Amersham, UK).
Western blot was conducted using a Multiphor II Electrophoresis System (GE Healthcare UK Ltd) at 200 mA for 1.30 h.
After the separation of the protein mixture, Western blot was conducted using the Trans-Blot® TurboTM Transfer System (Bio-Rad, USA).
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