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The blots were visualized using Immobilon Western (Millipore), and the intensity of the blots was analyzed using a Gel-Pro Analyzer version 4.0 (Media Cybernetics, Bethesda, MD, USA).
The images taken were assembled using Adobe Photoshop and band intensity on the Western blots was analyzed using ImageJ.
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Northern blots were analyzed using BAS 3000 (FujiFilm, Tokyo, Japan).
Total proteins were separated by SDS gel electrophoresis, and western blots were analyzed using the antiserum to TuMV CP.
The blots were analyzed using the LICOR Odyssey Infrared Imaging System, and the signals of the target proteins were normalized to the signal of β-actin which acts as a house-keeping protein.
Blots were analyzed using an Odyssey imaging system.
After extensive washing, the blots were analyzed using the LI-COR Odyssey infrared imaging system (Biosciences).
Northern blots were analyzed using a FLA3000 (FUJI) or a LAS Reader 3000 (FUJI).
Biochemical protein levels (western blots) were analyzed using two-tailed Student's t-tests to compare differences between two groups.
Western blots were analyzed using the Odyssey® infrared imaging system determining integrated intensities, using β-actin as a normalization control as described previously [29].
Data from quantitative western blots were analyzed using two-way ANOVA, followed by the Student-Newman-Keuls Method for pairwise multiple comparisons, as appropriate.
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