Sentence examples for blots in order to from inspiring English sources
We performed Western blots in order to: (i) Confirm that FOXO1a protein expression level is elevated in human compared to chimpanzees, and (ii) confirm the FOXO1a knockdown in HepG2/C3A liver cells (see below).
To ascertain that the PTS phosphorylation cascade is also functional in vivo we used Western blots in order to demonstrate the presence of P∼EIIANtr in B. melitensis crude extracts.
Band densities of acetylation specific blots were normalized to the respective densities of total histone H3 blots in order to obtain specific acetylation rates for each sample.
BACs isolated from library screening were subjected to a second round of screening via dot blots in order to determine which BACs were positive for each gene.
In a seminal investigation of a synthetic maize (Zea mays) autopolyploid series, Guo et al. (1996) established that rRNA exhibits a 1 1 dosage effect in response to changes in ploidy, then used rRNA as a loading control for northern blots in order to determine dosage responses for 18 genes.
We circumvented this complication by performing 2D gel electrophoresis combined with Western blotting in order to monitor possible changes to the isoelectric points of transport components.
For Aplysia Western blots, aliquots of samples for 2-DE were used for Western blotting in order to verify observed expressional differences.
Immunohistochemistry was done using the same specific antibodies as those for Western blotting, in order to support the data obtained by Western blotting as described previously [ 21, 23].
p53 protein levels were assessed by Western blot in order to confirm accumulation of this tumour suppressor in response to the BaP concentrations used in this study.
Protein extracts from non-transduced cultures were also submitted to western blot in order to discard changes in LC3-II level after lentiviral transduction; no differences were observed.
