An identical hybridisation pattern can be seen for the parB blot in comparison to the tcsL blot for the toxigenic C. sordellii isolates and the negative control R28058, however, a band can also be seen in the parB blot for UMC2 (Fig. 3B), confirming the presence of plasmid pCS1-4 in this strain.
The mutant proteins were expressed in COS-1 cells and analysed by western blotting in comparison to their WT counterpart: the DSC2 WT protein was detected predominantly in its cleaved, mature form ('M' in Figure 2 B, ∼90% Figure 2 C) and only small amounts of the un-cleaved precursor form could be detected ('P' in Figure 2 B, ∼10%, Figure 2 C; see Supplementary material online, Figure S2 ).
The simultaneous occurrence of many PbSERA3 peptides in Western blot experiments in comparison to relatively limited number of fragments in Western blot analysis of P. falciparum cell extracts (Li et al., 2002) reflects most likely the non-synchronous growth of P. berghei in mice.
Western blot studies showed that, in comparison to classical BSE (C-type), they are characterised by a higher or lower molecular mass of the unglycosylated PrPres and were thus named H-type and L-type BSE [ 20].
Proteins were quantified by measuring the density of the band on a western blot or Coomassie stained bands in comparison to a known protein concentration standard, using GeneTools software (SYNGENE) on scanned images.
The phenomenon that microarrays often provide compressed measurements in comparison to Northern blots or real time PCR is well known, however, its exact cause is not clear.
RhCD25 was characterized for expression by flow cytometric analyses, ELISA, Western blotting, functional signalling, and biological assays in comparison to HuCD25.
In comparison to Western blot, different CB-antibodies were chosen due to the incompatibility of the antibodies used during Western blot with IHC technology.
E-cadherin expression was analyzed by Western blotting in the parental subclone Caco-2-TC7 in comparison to a cell clone stably expressing meprinβ (Caco-2-β21).
After analysis by western blot (Figure 7) it was clear that in comparison to the manganese treated samples, the metal free and copper treated samples were more degraded than the wild-type protein (compare Figure 5).
