Sentence examples for blot without from inspiring English sources

Conditions for multiple heat shocks were determined empirically for each genotype to maximize target-gene silencing (determined by western blot) without causing lethality.

In order to know whether these receptors were located in the cell surface, live capacitated rabbit spermatozoa were incubated with the antibody used for western blot, without cell permeabilization.

Affinity captured MIF was not detectable by Western blot without concentration of the eluted fraction (Fig. 3C, Denatured, Elution, Elution conc).

The infrared imaging system allows detection of multiple proteins on a single blot, without the need for stripping and re-probing.

Below is a blot without BafA showing that basal levels are a little too low to clearly show a further reduction.

Six-hour treatments of both RWGT2 and HARA cells with 1  μ M PD resulted in a reduction of phosphorylated ERK1/2 as measured by Western blot without a change in the total ERK protein levels.

The relative band intensity was quantified as described in: http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html.html

The slides were blotted without washing and primary Abs were applied.

Therefore, relatively high levels of ERK1/2 phosphorylation were observed in western blots without performing additional bFGF stimulation, which was inevitable for ERK assay in mouse iPSCs.

This method is analogous to Western blotting without the need for dozens of antibodies and potential improvement in performance characteristics (Maiolica et al., 2012).

The serum from the final bleeding (87 days after first immunization) was used as antiserum for Western blot detection, without isolation of the IgG fraction.