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Proteins on the blot were visualized by enhanced chemiluminescence using the Lumi-lightPLUS Western blotting substrate (Roche), detected in an Epi Chemi II Darkroom equipped with a 12-bit SensiCam CCD camera and quantified using the Labworks 4.0 program (UVP BioImaging systems, Cambridge, United Kingdom).

The blot was visualized by HRP reaction.

The blot was visualized by enhanced chemiluminescence.

The blot was visualized by chemiluminescent detection (Pierce).

The western blot was visualized by enhanced chemiluminescence (Western Lightning-ECL Plus, PerkinElmer) and exposed to film.

The membrane was washed three times with TBST, and the blot was visualized by chemiluminescence via the Pierce SuperSignal ECL detection kit (Pierce, Thermo Fisher, USA).

The blot was visualized by exposure to a Storage Phosphor Screen (Amersham Biosciences, Piscataway, NJ) which was then scanned using a Storm 840 imaging system (Amersham Biosciences).

The blots were visualized by ECL GE HealthcareECL GE Healthcare

All blots were visualized by Odyssey infrared system (LI-COR Bioscience, Lincoln, Nebraska).

Proteins in the blots were visualized by SuperSignal West Pico Chemiluminescent Substrate (Pierce Chemical).

All blots were visualized by enhanced chemiluminescence using products from Pierce.