Sentence examples for blot was subsequently washed from inspiring English sources

The blot was subsequently washed 3× for 30 minute at RT with TBST followed by incubation with an anti-mouse-HRP secondary antibody (400 μg/ml dilution in TBST; Pierce, Rockford, IL) for 2 h at RT.

The blot was subsequently washed by 3 incubations with 1 mM methionine and 0,25% fatty-acid free BSA for at least 15 min, dried and binding quantified by phospho-imaging (Reader FLA 3000, Fuji-Film, Tokyo, Japan).

The blots were subsequently washed with TBST and incubated with respective secondary antibodies.

The blots were subsequently washed three times (10 15 min at RT) in TBST and once in TBS (5 10 min).

The blots were subsequently washed with TBS containing 1% Tween 20 for 4 times and then incubated with HRP-labeled goat anti-rabbit secondary antibody (1∶2000) (Boster, China) for 2 hours at room temperature.

For detection of IgG, the blots were subsequently washed in PBST and then incubated with rabbit anti-human IgG (diluted 1∶3000) (BIO-RAD, Hercules, CA) for one hour at 37°C.

Blots were subsequently washed 5 minutes in AP-buffer (0.1 M Tris Base, 0.1 M NaCl, pH9.5) and signal was detected on X-Ray film using CDP-star kit (Roche #12041677001) according to manufacturer's instructions with a typical exposure time of 4 hours.

The blots were subsequently washed and treated with appropriate secondary antibodies.

Blots were subsequently washed 3x10mins 0.1% Tween-PBS and GP73 visualized using an IRDye™ 700 Conjugated mouse anti-rabbit secondary antibody (1 10,000).

The blots were subsequently washed 3 times in 2 × SSC 0,1%SDS RT and twice 10 min in 0,1 × SSC 0,1%SDS at 65°C.

The blots were subsequently washed and then incubated with an appropriate horseradish peroxidase-conjugated secondary antibody, goat anti-rabbit IgG (Sigma).